carrier free recombinant mouse osteopontin  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Osteopontin from monocyte-derived dendritic cells mediates ozone-induced pulmonary responses in mice

doi: 10.3389/fimmu.2026.1748667

Figure Lengend Snippet: Cell counts in bronchoalveolar lavage fluid (BALF), airway hyperresponsiveness, and concentrations of osteopontin in lungs of mice exposed to air or ozone. (A) Experimental protocol. (B) Results of cell counts in BALF in mice exposed to air or ozone. (C) Lung histological examination by hematoxylin and eosin staining. (D) Results of airway hyperresponsiveness in mice exposed to air or ozone. (E) Concentrations of osteopontin in mice exposed to air or ozone. The data shown are pooled from multiple experiments (n = 5–7 per group). Results are means ± standard error. **p < 0.01, *p < 0.05 compared with mice exposed to air.

Article Snippet: In addition, 1.0x10 6 bone marrow neutrophils were stimulated with 1.0 μg/mL of mouse recombinant osteopontin (R&D Systems) in a 24-well dish for 2 hours, referring to a previous report , and collected for further RNA extraction and flowcytometric analyses.

Techniques: Staining

Journal: Frontiers in Immunology

Article Title: Osteopontin from monocyte-derived dendritic cells mediates ozone-induced pulmonary responses in mice

doi: 10.3389/fimmu.2026.1748667

Figure Lengend Snippet: Cell counts of monocyte-derived dendritic cells and macrophages in lungs, osteopontin concentrations in lungs, cell counts in bronchoalveolar lavage fluid (BALF), and airway hyperresponsiveness in mice treated with control liposome or clodronate liposome and exposed to air or ozone. (A) Experimental protocol. (B) Flowcytometric analysis of single lung cells in mice treated with control liposome or clodronate liposome. (C, D) Ratios of moDCs and macrophages in lungs of mice treated with control liposome or clodronate liposome. (E) Concentrations of osteopontin in lungs of mice treated with control liposome or clodronate liposome and exposed to air or ozone. (F) Cell counts in BALF and (G) airway hyperresponsiveness in mice treated with control liposome or clodronate liposome and exposed to air or ozone. The data shown are pooled from multiple experiments (n = 4–6 per group). Results are means ± standard error. *p < 0.05, **p < 0.01 compared with mice exposed to air. †p < 0.05, ††p < 0.01 compared with mice exposed to ozone and treated with control liposome. FCM, flow cytometry; FSC, forward scatter; SSC, side scatter; moDC, monocyte-derived dendritic cell; cont.lipo, control liposome; CL.lipo, clodronate liposome.

Article Snippet: In addition, 1.0x10 6 bone marrow neutrophils were stimulated with 1.0 μg/mL of mouse recombinant osteopontin (R&D Systems) in a 24-well dish for 2 hours, referring to a previous report , and collected for further RNA extraction and flowcytometric analyses.

Techniques: Derivative Assay, Control, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Osteopontin from monocyte-derived dendritic cells mediates ozone-induced pulmonary responses in mice

doi: 10.3389/fimmu.2026.1748667

Figure Lengend Snippet: Cell counts in bronchoalveolar lavage fluid (BALF) and airway hyperresponsiveness in mice treated with placebo or osteopontin inhibitor and exposed to air or ozone. (A) Experimental protocol. (B) Cell counts in BALF of mice treated with placebo or osteopontin inhibitor and exposed to air or ozone. (C) Airway hyperresponsiveness in mice treated with placebo or osteopontin inhibitor and exposed to air or ozone. The data shown are pooled from multiple experiments (n = 4–6 per group). Results are means ± standard error. *p < 0.05, **p < 0.01 compared with mice exposed to air. ††p < 0.01 compared with mice exposed to ozone and treated with placebo.

Article Snippet: In addition, 1.0x10 6 bone marrow neutrophils were stimulated with 1.0 μg/mL of mouse recombinant osteopontin (R&D Systems) in a 24-well dish for 2 hours, referring to a previous report , and collected for further RNA extraction and flowcytometric analyses.

Techniques:

Journal: Frontiers in Immunology

Article Title: Osteopontin from monocyte-derived dendritic cells mediates ozone-induced pulmonary responses in mice

doi: 10.3389/fimmu.2026.1748667

Figure Lengend Snippet: RNA sequencing and protein analyses of bone marrow neutrophils stimulated by osteopontin. (A) Experimental protocol of purification of bone marrow neutrophils. (B) Morphological analysis of bone marrow neutrophils by Diff-Quik staining. (C) Principal component scores with the confidence ellipsoids in bone marrow neutrophils with or without stimulation by osteopontin. (D) Volcano plots for differential expression analysis in bone marrow neutrophils with or without stimulation by osteopontin. (E) Gene expression of NDUFA1 in bone marrow neutrophils with or without stimulation by osteopontin. The data shown are pooled from 6 samples per group. Results are means ± standard error. *p < 0.05 compared with the result of bone marrow neutrophils without stimulation.

Article Snippet: In addition, 1.0x10 6 bone marrow neutrophils were stimulated with 1.0 μg/mL of mouse recombinant osteopontin (R&D Systems) in a 24-well dish for 2 hours, referring to a previous report , and collected for further RNA extraction and flowcytometric analyses.

Techniques: RNA Sequencing, Purification, Diff-Quik, Staining, Quantitative Proteomics, Gene Expression